Starting year 2021

Nagoya University
Graduate School of Medicine
Associate Professor

Research Areas:Life Science

Research fields

Developmental biology

Research Interests

Brain development
Glia, Maternal inflammation

Professional Memberships

The Japanese Association of Anatomists
The Japan Neuroscience Society
The Japanese Society for Neurochemistry
The Molecular Biology Society of Japan
The Japanese Society of Developmental Biologists
Society for Neuroscience

Main research topics

Neocortical development proceeds through precisely controlled various steps, which organize the positioning of neural-lineage cells based on their differentiation status in the developing brain. Neural stem cells, which are born at near the ventricular surface, make a fate choice of either repeating cell division to keep their immature state or differentiation, and the fated cells toward the differentiation mature into neurons, astrocytes, and oligodendrocytes with their migration toward the outside (basal side).
Previous studies aimed at investigating such mechanisms have been focused on only neural-lineage cells, but microglia, the resident immune cells in the central nervous system, have been recently reported to be involved in these steps. For example, microglia promote the differentiation of neural progenitor cells, regulate their numbers, and control the localization of neurons in the embryonic brain.
Interestingly, microglia change their distribution in the developing cerebral wall in a stage-dependent manner: they disappear from the cortical plate in the mid embryonic stage. We demonstrated that molecular mechanisms of microglial migration in the cerebral wall, and their transient absence from the cortical plate is required for postmigratory neurons to appropriately fine-tune the expression of molecules needed for proper differentiation (1).
On the other hand, microglia also contribute to vascular formation and branching. We recently reported that pericytes, the mural cells surrounding endothelial cells, facilitate microglial homeostasis and survival in the developing brains, thereby indirectly supporting microglial effects on neural progenitors, such as promoting the differentiation (2).
Recent single-cell transcriptome analyses revealed that microglia have a heterogeneity on their gene expression patterns not only in the adult brain but also in the embryonic stage. We are now investigating which factors induce microglial diversity, focusing on the environmental factors in the brain and microglial distribution routes or timing to reach the brain. We also try to understand the significance of the interaction between various cell types, such as microglia, surrounding neural lineage cells and vasculature forming cells through in vivo live imaging system.

Representative papers

Hattori Y, Naito Y, Tsugawa Y, Nonaka S, Wake H, Nagasawa T, Kawaguchi A, Miyata T. Transient microglial absence assists postmigratory neurons in proper differentiation. Nat. Commun. 11, 1631 (2020).

Hattori Y, Itoh H, Tsugawa Y, Nishida Y, Kurata K, Uemura A, Miyata T. Embyonic pericytes promote microglial homeostasis and their effects on neural progenitors in the developing cortex. J. Neurosci. 42 (3), 362–376 (2022).

Research URL

researchmap https://researchmap.jp/yukihattori

Laboratory Website https://www.takaki-miyata-lab.org

Topics of Neurochemistry (Articles on previous research) https://www.neurochemistry.jp/mu0czb0qu-15/#_15

Global issues to be solved through this project

The influence of maternal inflammation on microglial activity and function in the developing brain

This project is aimed at dissecting the roles of embryonic microglia in the brain both in the physiological and pathological state. We will first analyze the correlation between microglial colonization routes reaching the brain and the their genetical and functional heterogeneity. Through developing the in vivo imaging technology for embryos, we try to understand the significance of cell-cell interaction between various cell type, such as microglia, neural lineage cells, and vasculature cells. Furthermore, how maternal immune activation by viral and bacterial infection, malnutrition, or stress, changes microglial properties in the fetal brain is our big interest. Thus, we will investigate the influence of such maternal inflammation on the nervous system development and vascular formation in the embryonic brain as the next step. Our goal through this project is to establish the platform for developing research for early diagnosis, prevention, and treatment of mental disorders, hearing impairment and other brain developmental abnormalities.